Bent and dent store
Plant classification 5th grade
Darwinpercent27s voyage of discovery quizlet
Cas9 Sgrna Expression Plasmid Px330, supplied by Addgene inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more pX330. The resulting vector contains a cytomegalovirus b-actin hybrid promoter driving iCas9 expression. Single guide RNA guides were synthesized as pairs of oligonucleotides. Terminal 5¢ phosphates were added to oligonucleotides by incubating 1lg total of top/bottom oligonucleotides in 50lL reactions containing 1·T4